To identify potential patients, a diagnostic workup of AIHA is essential, including antibody type, patient history and physical exam, hemoglobin levels and blood cell counts, blood smear examination and direct antiglobulin test (DAT)
Diagnostic criteria for CAD include chronic hemolysis, polyspecific DAT positive, monospecific DAT strongly positive for C3d, CA titer ≥64 at 4°C and a bone marrow evaluation (advised in patients with CAD at diagnosis)
Hemoglobin is the most direct indicator of clinical severity
