3 Minute 3Rs

Feb 16 2023 18
3 Minute 3Rs Podcast artwork

3 Minute 3Rs is your monthly recap of efforts to replace, reduce, and refine the use of animals in research, brought to you by the NC3Rs, the North American 3Rs Collaborative, and Lab Animal.
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Assessing enrichment, consolidating animal research guidance and understanding mouse aggression

Feb 16 2023 4 mins  
February 2023 The papers behind the pod: Hobbiesiefken U et al. (2023). Rating enrichment items by female group-housed laboratory mice in multiple binary choice tests using an RFID-based tracking system. PloS one 18(1): e0278709. doi: 10.1371/journal.pone.0278709Petkov C et al. (2022). Unified ethical principles and an animal research �

Replacing animal-derived reagents, simulating in utero microinjections and clicker training for mouse gait assessment

Jan 19 2023 4 mins  
January 2023 The papers behind the pod: Cassotta M et al. (2022). A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation. Engineering in Life Sciences 22(9):561-604. doi: 10.1002/elsc.202100167  Nuber M et al. (2022). Development of a 3D simulator for training the mouse in uter

Kidney organoid vascularisation, implementing masking and quantitatively assessing experiment severity

Dec 14 2022 4 mins  
December 2022The papers behind the pod: Menéndez ABC et al. (2022). Creating a kidney organoid-vasculature interaction model using a novel organ-on-chip system. Scientific Reports 12:20699. doi: 10.1038/s41598-022-24945-5Karp N et al. (2022). A qualitative study of the barriers to using blinding in in vivo experiments and sugg

Assessing pain, living systematic reviews and inducing focal hypoxia in human neurons

Nov 17 2022 4 mins  
November 2022The papers behind the pod: Aulehner K et al. (2022). Grimace scale, burrowing, and nest building for the assessment of post-surgical pain in mice and rats—A systematic review. Frontiers in Veterinary Science 9:930005. doi: 10.3389/fvets.2022.930005Hair K et al. (2022). ‘Living’ evidence frameworks for in vivo anim

Statistical planning, human cell cultures for toxoplasma and preventing boredom in laboratory rodents

Oct 20 2022 4 mins  
October 2022The papers behind the pod: Piper, SK et al. (2022). Statistical review of animal trials—A guideline. Biometrical Journal https://doi.org/10.1002/bimj.202200061 Gargaté MJ et al. (2022). Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model. Pathogens https://doi.org/10.3390/pa

Larger species refinement special: improving the welfare of rabbits, non-human primates and sheep

Sep 15 2022 4 mins  
September 2022The papers behind the pod: Pinho RH et al. (2022). Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus). PLoS ONE 17(5): e0268973. https://doi.org/10.1371/journal.pone.0268973 Stull C, Heagerty A and Coleman K (2022). Video Conference Technology as a Tool for Pair Introducti

Better behavioural research, imaging with microbots and how housing density affects mouse microbiomes

Aug 18 2022 4 mins  
August 2022The papers behind the pod: Arjmand S et al. (2022). Tips and traps for behavioural animal experimentation. Acta Neuropsychiatrica, in press. https://doi.org/10.1017/neu.2022.4Wrede P et al. (2022). Real-time 3D optoacoustic tracking of cell-sized magnetic microrobots circulating in the mouse brain vasculature. Science Advances

3Rs Prize: A benchtop organ-on-a-chip fabrication method and an ex vivo model of focal demyelination

Jul 21 2022 4 mins  
July 2022The papers behind the pod: Ferreira DA et al. (2021). Alternative to Soft Lithography for the Fabrication of Organ-on-a-Chip Elastomeric-Based Devices and Microactuators. Advanced Science 8:2003273.  https://doi.org/10.1002/advs.202003273Eigel D et al. (2019). Cryogel scaffolds for regionally constrained delivery of lysophosphatidylcholine to centr

Humane intervention points, virtual gene knockout and ex vivo brain slices for Parkinson's research

Jun 16 2022 4 mins  
June 2022The papers behind the pod: Williams WO and Baneux P (2022). Humane Intervention Points: Refining endpoint terminology to incorporate non-euthanasia intervention options to improve animal welfare and preserve experimental outcomes. Laboratory Animals, in press. https://doi.org/10.1177/00236772221090801 Osorio D et al. (2022). scTenifoldKnk: An effic

Microbrains for neurotoxicity testing, improved experimental design, and post-op severity assessment

May 19 2022 4 mins  
May 2022 The papers behind the pod:  Wang, Q et al. (2022). Assessment of a 3D neural spheroid model to detect pharmaceutical-induced neurotoxicity. ALTEX 39. https://doi.org/10.14573/altex.2112221 Reynolds P.S. (2022) Between two stools: preclinical research, reproducibility, and statistical design of experiments. BMC Res. Notes 15, 73.